Holub, E., Hondl, N., Ramer, G., & Lendl, B. (2024). Photothermal Spectroscopy: System Design and Applications. In ICPPP22 International Conference on Photoacoustic and Photothermal Phenomena: Book of Abstracts (pp. 303–305).
Optical photothermal infrared (O-PTIR) spectroscopy combines a resolution at the visible diffraction limit,
biological specificity and non-contact sample analysis [1]. Although these characteristics make it an ideal
candidate for the analysis of biological specimens, evidence of O-PTIR experiments on cells and tissues in
aqueous environments is limited ([2], [3]), and the O PTIR technique has not yet been exploited for the
analysis of samples within organ-on-a-chip (OOC) systems. A dedicated O-PTIR spectroscope was developed at TU
Wien for the analysis of biological samples in water filled channels. Photothermal spectra and images of
endothelial and cancer cells were recorded in both air and water for subsequent qualitative and quantitative
analysis.
[1] Adhikari, S., Spaeth, P., Kar, A., Baaske, M. D., Khatua, S. and and Orrit, M. “Photothermal Microscopy: Imaging
the Optical Absorption of Single Nanoparticles and Single Molecules”. In: ACS Nano 14 (2020), pp. 16414–16445.
[2] Mërtiri, A., Totachawattana, A., Liu, H., Hong, M. K., Gardner, T., Sander, M. and Erramilli, S. “Label Free Mid-IR
Photothermal Imaging of Bird Brain With Quantum Cascade Laser”. In: Conference on Lasers and Optics, San Jose,
CA, USA (2014), pp. 1–2.
[3] Zhang, D., Li, C., Zhang, C., Slipchenko, M. N., Eakins, G. and Cheng, J.-X. (2016) “Depth-resolved mid-infrared
photothermal imaging of living cells and organisms with submicrometer spatial resolution”. In: Sci. Adv.
2(9):e1600521.
en
Project title:
Tumor und Lymphknoten auf einer Chip Plattform für Krebsstudien: 953234 (European Commission)
