Nanomotif: Identification and Exploitation of DNA Methylation Motifs in Metagenomes using Oxford Nanopore Sequencing

Heidelbach, Søren; Mølvang Dall, Sebastian; Bøjer, Jeppe Støtt; Nissen, Jacob; van der Maas, Lucas N.L.; Sereika, Mantas; Kirkegaard, Rasmus H.; Jensen, Sheila I.; Kousgaard, Sabrina Just; Thorlacius-Ussing, Ole; Hose, Katja; Nielsen, Thomas Dyhre; Albertsen, Mads

doi:10.1101/2024.04.29.591623

Record link:

http://hdl.handle.net/20.500.12708/208546
https://doi.org/10.34726/8211

Title:

Nanomotif: Identification and Exploitation of DNA Methylation Motifs in Metagenomes using Oxford Nanopore Sequencing

Citation:

Heidelbach, S., Mølvang Dall, S., Bøjer, J. S., Nissen, J., van der Maas, L. N. L., Sereika, M., Kirkegaard, R. H., Jensen, S. I., Kousgaard, S. J., Thorlacius-Ussing, O., Hose, K., Nielsen, T. D., & Albertsen, M. (2024). Nanomotif: Identification and Exploitation of DNA Methylation Motifs in Metagenomes using Oxford Nanopore Sequencing. bioRxiv. https://doi.org/10.34726/8211

reposiTUm DOI:

10.34726/8211

CatalogPlus:

AC17407979

Publisher DOI:

10.1101/2024.04.29.591623

Publication Type:

Preprint

Language:

English

Organisational Unit:

E192-02 - Forschungsbereich Databases and Artificial Intelligence

Date (published):

2-May-2024

Number of Pages:

Preprint Server:

bioRxiv

Keywords:

DNA methylation; microbial biology; metagenome-assembled genomes (MAGs)

Abstract:

DNA methylation is vital for understanding microbial biology, but a rarely used feature in recovery of metagenome-assembled genomes (MAGs). Recently, Oxford Nanopore introduced all context methylation detection models. We leveraged this to develop Nanomotif - a tool for identification of methylated motifs in metagenomic contigs. We demonstrate how this enables MAG contamination detection, association of mobile genetic elements, and linking of motifs with the responsible methyltransferase directly from Nanopore data.

Project title:

Health virtual twins for the personalised management of stroke related to atrial fibrillation: ??? (European Commission)

Research Areas:

Logic and Computation: 100%

Science Branch:

1020 - Informatik: 80%
1010 - Mathematik: 20%

License:

CC BY 4.0