Imaging a specimen using complementary modalities enables the generation of an integrated, multiscale representation that captures structural and molecular information across relevant resolution ranges. Here, we present a series of sample preparation workflows designed to combine multiple imaging technologies with MALDI mass spectrometry imaging (MSI), enabling the acquisition of spatially resolved molecular data and facilitating a more comprehensive characterization of biological tissues. This multimodal strategy enhances the interpretation of tissue morphology by linking structural features to their underlying molecular composition. We introduce a multimodal imaging workflow integrating infrared (IR), Raman spectroscopy, and MSI to investigate lipid and protein alterations in tissue exposed to environmental stressors such as ultraviolet (UV) radiation and sulfur dioxide (SO₂). Spectroscopic analyses reveal that tissue damage is associated with three-dimensional structural rearrangements and chemical modifications of proteins and lipids. MSI gives details for modifications on the molecular level. In a second application, we present a correlative multimodal approach that links microscale biomechanics to protein composition in human meniscus tissue. By carefully optimizing sample handling and measurement conditions, fresh tissue can be analyzed using atomic force microscopy (AFM) micro-indentation and MSI on the same section. This enables the assessment of local nanometer-scale bioelastic properties with collagen distribution mapped across the entire tissue at micrometer lateral resolution.