Title: External cavity-quantum cascade laser (EC-QCL) spectroscopy for protein analysis in bovine milk
Language: English
Authors: Kuligowski, Julia 
Schwaighofer, Andreas 
Alcaráz, Mirta Raquel 
Quintás, Guillermo
Mayer, Helmut
Vento, Maximo 
Lendl, Bernhard  
Keywords: External cavity-quantum cascade laser (ECQCL) spectroscopy; Mid-infrared (IR); Protein analysis; Milk analysis; Science-based calibration (SBC)
Issue Date: 2017
Journal: Analytica Chimica Acta
Abstract: 
he analytical determination of bovine milk proteins is important in food and non-food industrial applications and yet, rather labour-intensive wet-chemical, low-throughput methods have been employed since decades. This work proposes the use of external cavity-quantum cascade laser (EC-QCL) spectroscopy for the simultaneous quantification of the most abundant bovine milk proteins and the total protein content based on the chemical information contained in mid-infrared (IR) spectral features of the amide I band. Mid-IR spectra of protein standard mixtures were used for building partial least squares (PLS) regression models. Protein concentrations in commercial bovine milk samples were calculated after chemometric compensation of the matrix contribution employing science-based calibration (SBC) without sample pre-processing. The use of EC-QCL spectroscopy together with advanced multivariate data analysis allowed the determination of casein, a-lactalbumin, b-lactoglobulin and total protein content within several minutes
DOI: 10.1016/j.aca.2017.02.003
Library ID: AC15518676
URN: urn:nbn:at:at-ubtuw:3-7544
ISSN: 0003-2670
Organisation: E164 - Institut für Chemische Technologien und Analytik 
License URI: https://creativecommons.org/licenses/by-nc-nd/4.0/deed.de
Publication Type: Article
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