<div class="csl-bib-body">
<div class="csl-entry">Alcaráz, M. R., Schwaighofer, A., Kristament, C., Ramer, G., Brandstetter, M., Goiccoechea, H., & Lendl, B. (2015). External cavity-quantum cascade laser spectroscopy for mid-IR transmission measurements of proteins in aqueous solution. <i>Analytical Chemistry</i>, <i>87</i>(13), 6980–6987. https://doi.org/10.1021/acs.analchem.5b01738</div>
</div>
The final publication is available via <a href="https://doi.org/10.1021/acs.analchem.5b01738" target="_blank">https://doi.org/10.1021/acs.analchem.5b01738</a>.
-
dc.description.abstract
In this work, we report mid-IR transmission measurements of the protein amide I band in aqueous solution at large optical paths. A tunable external-cavity quantum cascade laser (EC-QCL) operated in pulsed mode at room temperature allowed one to apply a path length of up to 38 μm, which is four times larger than that applicable with conventional FT-IR spectrometers. To minimize temperature-induced variations caused by background absorption of the ν2-vibration of water (HOH-bending) overlapping with the amide I region, a highly stable temperature control unit with relative temperature stability within 0.005 °C was developed. An advanced data processing protocol was established to overcome fluctuations in the fine structure of the emission curve that are inherent to the employed EC-QCL due to its mechanical instabilities. To allow for wavenumber accuracy, a spectral calibration method has been elaborated to reference the acquired IR spectra to the absolute positions of the water vapor absorption bands. Employing this setup, characteristic spectral features of five well-studied proteins exhibiting different secondary structures could be measured at concentrations as low as 2.5 mg mL−1. This concentration range could previously only be accessed by IR measurements in D2O. Mathematical evaluation of the spectral overlap and comparison of second derivative spectra confirm excellent agreement of the QCL transmission measurements with protein spectra acquired by FT-IR spectroscopy. This proves the potential of the applied setup to monitor secondary structure changes of proteins in aqueous solution at extended optical path lengths, which allow experiments in flow through configuration.
en
dc.description.sponsorship
Austrian Research Promotion Agency (FFG)
-
dc.language
English
-
dc.language.iso
en
-
dc.publisher
American Chemical Society
-
dc.relation.ispartof
Analytical Chemistry
-
dc.rights.uri
http://rightsstatements.org/vocab/InC/1.0/
-
dc.title
External cavity-quantum cascade laser spectroscopy for mid-IR transmission measurements of proteins in aqueous solution
en
dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Urheberrechtsschutz
de
dc.rights.license
In Copyright
en
dc.contributor.affiliation
Ciudad Universitaria, Santa Fe, Argentina
-
dc.description.startpage
6980
-
dc.description.endpage
6987
-
dc.relation.grantno
843546
-
dc.rights.holder
American Chemical Society 2016
-
dc.type.category
Original Research Article
-
tuw.container.volume
87
-
tuw.container.issue
13
-
tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
tuw.version
smur
-
wb.publication.intCoWork
International Co-publication
-
dcterms.isPartOf.title
Analytical Chemistry
-
tuw.publication.orgunit
E164 - Institut für Chemische Technologien und Analytik
-
tuw.publisher.doi
10.1021/acs.analchem.5b01738
-
dc.identifier.eissn
1520-6882
-
dc.identifier.libraryid
AC15520864
-
dc.identifier.urn
urn:nbn:at:at-ubtuw:3-7633
-
tuw.author.orcid
0000-0003-2714-7056
-
tuw.author.orcid
0000-0001-8307-5435
-
tuw.author.orcid
0000-0003-3838-5842
-
dc.rights.identifier
Urheberrechtsschutz
de
dc.rights.identifier
In Copyright
en
wb.sci
true
-
item.fulltext
with Fulltext
-
item.grantfulltext
open
-
item.languageiso639-1
en
-
item.openairetype
research article
-
item.cerifentitytype
Publications
-
item.openaccessfulltext
Open Access
-
item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
-
crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
-
crisitem.author.dept
Ciudad Universitaria, Santa Fe, Argentina
-
crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.orcid
0000-0003-2714-7056
-
crisitem.author.orcid
0000-0001-8307-5435
-
crisitem.author.orcid
0000-0003-3838-5842
-
crisitem.author.parentorg
E150 - Fakultät für Technische Chemie
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E150 - Fakultät für Technische Chemie
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik