Time-Resolved Surface-Enhanced IR-Absorption Spectroscopy of Direct Electron Transfer to Cytochrome c Oxidase from R. sphaeroides

Schwaighofer, Andreas; Steininger, Christoph; Hildenbrandt, David; Srajer, Johannes; Nowak, Christoph; Knoll, Wolfgang; Naumann, Renate

doi:10.1016/j.bpj.2013.10.037

Record link:

https://resolver.obvsg.at/urn:nbn:at:at-ubtuw:3-7610
http://hdl.handle.net/20.500.12708/697

Title:

Time-Resolved Surface-Enhanced IR-Absorption Spectroscopy of Direct Electron Transfer to Cytochrome c Oxidase from R. sphaeroides

Citation:

Schwaighofer, A., Steininger, C., Hildenbrandt, D., Srajer, J., Nowak, C., Knoll, W., & Naumann, R. (2013). Time-Resolved Surface-Enhanced IR-Absorption Spectroscopy of Direct Electron Transfer to Cytochrome c Oxidase from R. sphaeroides. Biophysical Journal. https://doi.org/10.1016/j.bpj.2013.10.037

Publisher DOI:

10.1016/j.bpj.2013.10.037

CatalogPlus:

AC15520862

Publication Type:

Article - Original Research Article

Language:

English

Authors:

Schwaighofer, Andreas
Steininger, Christoph
Hildenbrandt, David
Srajer, Johannes
Nowak, Christoph
Knoll, Wolfgang
Naumann, Renate

Organisational Unit:

E164 - Institut für Chemische Technologien und Analytik

Journal:

Biophysical Journal

ISSN:

0006-3495

Date (published):

17-Dec-2013

Publisher:

Cell Press

Peer reviewed:

Yes

Abstract:

Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.

Additional information:

The final publication is available via <a href="https://doi.org/10.1016/j.bpj.2013.10.037" target="_blank">https://doi.org/10.1016/j.bpj.2013.10.037</a>.

License:

In Copyright

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Article