Title: Time-Resolved Surface-Enhanced IR-Absorption Spectroscopy of Direct Electron Transfer to Cytochrome c Oxidase from R. sphaeroides
Language: English
Authors: Schwaighofer, Andreas  
Steininger, Christoph 
Hildenbrandt, David 
Srajer, Johannes
Nowak, Christoph
Knoll, Wolfgang
Naumann, Renate
Category: Original Research Article
Issue Date: 17-Dec-2013
Journal: Biophysical Journal
ISSN: 0006-3495
Abstract: 
Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.
DOI: 10.1016/j.bpj.2013.10.037
Library ID: AC15520862
URN: urn:nbn:at:at-ubtuw:3-7610
Organisation: E164 - Institut für Chemische Technologien und Analytik 
Publication Type: Article
Artikel
Appears in Collections:Article

Files in this item:


Page view(s)

130
checked on Oct 2, 2021

Download(s)

65
checked on Oct 2, 2021

Google ScholarTM

Check


Items in reposiTUm are protected by copyright, with all rights reserved, unless otherwise indicated.