Title: Time-Resolved Surface-Enhanced IR-Absorption Spectroscopy of Direct Electron Transfer to Cytochrome c Oxidase from R. sphaeroides
Language: English
Authors: Schwaighofer, Andreas  
Steininger, Christoph 
Hildenbrandt, David 
Srajer, Johannes
Nowak, Christoph
Knoll, Wolfgang
Naumann, Renate
Category: Original Research Article
Issue Date: 17-Dec-2013
Journal: Biophysical Journal
ISSN: 0006-3495
Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.
DOI: 10.1016/j.bpj.2013.10.037
Library ID: AC15520862
URN: urn:nbn:at:at-ubtuw:3-7610
Organisation: E164 - Institut für Chemische Technologien und Analytik 
Publication Type: Article
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