Schwaighofer, A., Steininger, C., Hildenbrandt, D., Srajer, J., Nowak, C., Knoll, W., & Naumann, R. (2013). Time-Resolved Surface-Enhanced IR-Absorption Spectroscopy of Direct Electron Transfer to Cytochrome c Oxidase from R. sphaeroides. Biophysical Journal. https://doi.org/10.1016/j.bpj.2013.10.037
E164 - Institut für Chemische Technologien und Analytik
Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.
The final publication is available via <a href="https://doi.org/10.1016/j.bpj.2013.10.037" target="_blank">https://doi.org/10.1016/j.bpj.2013.10.037</a>.