Title: Valorisation of cheese whey as substrate and inducer for recombinant protein production in E. coli HMS174(DE3)
Language: English
Authors: Hausjell, Johanna 
Miltner, Martin  
Herzig, Christopher  
Limbeck, Andreas  
Saracevic, Zdravka 
Saracevic, Ernis 
Weissensteiner, Julia 
Molitor, Christian 
Halbwirth, Heidrun  
Spadiut, Oliver 
Category: Original Research Article
Issue Date: 2019
Hausjell, J., Miltner, M., Herzig, C., Limbeck, A., Saracevic, Z., Saracevic, E., Weissensteiner, J., Molitor, C., Halbwirth, H., & Spadiut, O. (2019). Valorisation of cheese whey as substrate and inducer for recombinant protein production in E. coli HMS174(DE3). Bioresource Technology Reports, 8, Article 100340. https://doi.org/10.1016/j.biteb.2019.100340
Journal: Bioresource Technology Reports 
Every year worldwide around 190 million tons of cheese whey are generated resulting in a huge environmental burden. We recently published a study where we showed that E. coli strain HMS174(DE3) can be cultivated using only lactose as C-source and inducer. Motivated by the results we investigated using a concentrated whey feed instead of the lactose feed. Spray drying whey and dissolving the powder allowed preparation of a 40-fold concentrated whey containing 91% lactose and 81% protein of the original whey. Cultivations using the concentrated whey feed instead of a defined lactose feed revealed 39% higher growth rates, 24% higher biomass yields and even higher specific product titers for the model enzymes, flavanone 3-hydroxylase and chalcone 3- hydroxylase. Our strategy simultaneously provides a cheap substrate for large-scale production of technical enzymes and an excellent opportunity for cheese whey valorization, reducing the biological burden resulting from whey wastewaters.
Keywords: Whey; Escherichia coli; HMS174(DE3); Sustainable recombinant protein production; Waste-to-value
DOI: 10.1016/j.biteb.2019.100340
Library ID: AC15590178
URN: urn:nbn:at:at-ubtuw:3-8574
Organisation: E226 - Institut für Wassergüte und Ressourcenmanagement 
E226-01 - Forschungsbereich Wassergütewirtschaft 
Publication Type: Article
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