Title: Binding kinetics between the T cell receptor and its ligand pMHC
Language: English
Authors: Dungl, Dominik 
Qualification level: Diploma
Keywords: single molecule microscopy; FRET; T cell; immunological synapse; off-rate
Advisor: Schütz, Gerhard 
Issue Date: 2019
Number of Pages: 45
Qualification level: Diploma
Abstract: 
The recognition of pathogens by the T cell receptor plays a crucial role in the immune system. The binding kinetics between the T cell receptor and its ligand are still debated. They have been characterized by Huppa et al. using a fluorescence microscopy combined with a Förster Resonant Energy Transfer (FRET) based approach and reported off times of approximately 0.1 s. In contrast ODonoghue et al. reported different off times of approximately 5 s using fluorescence microscopy with long exposure times. In this thesis, it was attempted to reconcile both results by combining the methods of both papers to measure the binding kinetics of T cell receptors and its ligands. Experimental procedures were established and receptorligand interactions were observed using long exposures, but the FRET experiments were inconclusive due to similar results in negative control experiments.
URI: https://resolver.obvsg.at/urn:nbn:at:at-ubtuw:1-128340
http://hdl.handle.net/20.500.12708/11427
Library ID: AC15455644
Organisation: E134 - Institut für Angewandte Physik 
Publication Type: Thesis
Hochschulschrift
Appears in Collections:Thesis

Files in this item:

Show full item record

Page view(s)

6
checked on Feb 18, 2021

Download(s)

12
checked on Feb 18, 2021

Google ScholarTM

Check


Items in reposiTUm are protected by copyright, with all rights reserved, unless otherwise indicated.