Title: Creating universality for cancer gene therapy in applying Adenovirus vectors with altered tropism : designing Adenovirus particles using K-Coil/E-Coil technology to control receptor specificity
Language: English
Authors: Kastberger, Birgit 
Qualification level: Diploma
Advisor: Gilbert, Rénald 
Assisting Advisor: Kubicek, Peter Christian
Gadek-Wesierski, Jozefa 
Issue Date: 2011
Number of Pages: 91
Qualification level: Diploma
For my Master Thesis project I was charged with the design of two recombinant types of adenoviruses. The first adenovirus should possess a K-coil sequence situated in the HI-loop region of the fibre's knob domain, the other one was supposed to have an ablated CAR tropism in addition to the identical K-coil insertion. The virus' transduction efficiency via EGF-receptors was monitored by fluorescents provoked by a GFP marker, that had to be inserted into the E1 region of the AdV genome for this reason.
Keywords: Adenovirus; Gentherapie; K-Coil; E-Coil Technologie; Krebs; Biotechnologie; Tropismus; Car-Rezeptor; GFP-Marker
Adenovirus; Gene therapy; K-Coil; E-Coil Technology; Cancer; Biotechnology; Tropism; Car-Receptor; GFP-Marker
URI: https://resolver.obvsg.at/urn:nbn:at:at-ubtuw:1-42163
Library ID: AC07809229
Organisation: E166 - Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften 
Publication Type: Thesis
Appears in Collections:Thesis

Files in this item:

Show full item record

Page view(s)

checked on Apr 1, 2021


checked on Apr 1, 2021

Google ScholarTM


Items in reposiTUm are protected by copyright, with all rights reserved, unless otherwise indicated.