| Title: | Creating universality for cancer gene therapy in applying Adenovirus vectors with altered tropism : designing Adenovirus particles using K-Coil/E-Coil technology to control receptor specificity | Language: | English | Authors: | Kastberger, Birgit | Qualification level: | Diploma | Advisor: | Gilbert, Rénald | Assisting Advisor: | Kubicek, Peter Christian Gadek-Wesierski, Jozefa |
Issue Date: | 2011 | Number of Pages: | 91 | Qualification level: | Diploma | Abstract: | For my Master Thesis project I was charged with the design of two recombinant types of adenoviruses. The first adenovirus should possess a K-coil sequence situated in the HI-loop region of the fibre's knob domain, the other one was supposed to have an ablated CAR tropism in addition to the identical K-coil insertion. The virus' transduction efficiency via EGF-receptors was monitored by fluorescents provoked by a GFP marker, that had to be inserted into the E1 region of the AdV genome for this reason. |
Keywords: | Adenovirus; Gentherapie; K-Coil; E-Coil Technologie; Krebs; Biotechnologie; Tropismus; Car-Rezeptor; GFP-Marker Adenovirus; Gene therapy; K-Coil; E-Coil Technology; Cancer; Biotechnology; Tropism; Car-Receptor; GFP-Marker |
URI: | https://resolver.obvsg.at/urn:nbn:at:at-ubtuw:1-42163 http://hdl.handle.net/20.500.12708/12301 |
Library ID: | AC07809229 | Organisation: | E166 - Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften | Publication Type: | Thesis Hochschulschrift |
| Appears in Collections: | Thesis |
Files in this item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Kastberger Birgit - 2011 - Creating universality for cancer gene therapy in...pdf | 3.32 MB | Adobe PDF |  View/Open |
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