Title: Atomic force microscopy of the alga Euglena gracilis
Language: English
Authors: Gruenberger, Clemens Christian 
Qualification level: Diploma
Keywords: Alge; Rasterkraftmikroskopie; Nanotechnologie; Bionanotechnologie; Biomaterialien; Euglena
algae; AFM; Atomic Force Microscopy; Nanotechnology; Bionanotechnology; Biomaterials; Euglena
Advisor: Aumayr, Friedrich 
Assisting Advisor: Gebeshuber, Ilse-Christine
Issue Date: 2007
Number of Pages: 109
Qualification level: Diploma
Abstract: 
In dieser Diplomarbeit wurde eine Methode entwickelt, um die Alge Euglena gracilis mittels Rasterkraftmikroskopie unter verschiedenen Bedingungen zu untersuchen. Daten wurden gesammelt von vollständigen Zellen, als auch von Zellbestandteilen. Einige der gefundenen Charakteristika werden mit Daten aus der Literatur verglichen (meist transmissionselektronenmikroskopische und rasterelektronenmikroskopische Aufnahmen). Die Möglichkeit rasterkraftmikroskopische Kraftkurven aufzunehmen und viskoelastische Studien durchzuführen wird demonstriert.
Der Ausblick zeigt verschiedene mögliche Richtungen zukünftiger Forschung auf, die den hier verfolgten Ansatz weiterführen.

Matter produced and assembled by even simple life forms is remarkable. Euglena gracilis, a single-celled algal species, performs tasks as diverse as sensing the environment and reacting to it, converting and storing energy and metabolizing nutrients, living as a plant or an animal depending on the environmental constraints. Cell functions are often based on materials produced with molecular precision. In this thesis, a method for Atomic Force Microscope investigation of the alga Euglena gracilis was developed and measurements under different scanning conditions were carried out. Data was obtained on whole cells as well as cell organelles. Some of the obtained morphological algal features were compared to existing literature data (mostly Transmission Electron Microscope and Scanning Electron Microscope images). The possibility of AFM force spectroscopy and viscoelastic analysis on the nanoscale concerning this biological system has been demonstrated. The outlook points at possible directions of future research, that extend the approach here presented.
URI: https://resolver.obvsg.at/urn:nbn:at:at-ubtuw:1-18472
http://hdl.handle.net/20.500.12708/13958
Library ID: AC05035826
Organisation: E134 - Institut für Allgemeine Physik 
Publication Type: Thesis
Hochschulschrift
Appears in Collections:Thesis

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