Kopp, J., Bayer, B., Slouka, C., Striedner, G., Dürkop, M., & Spadiut, O. (2022). Fundamental insights in early-stage inclusion body formation. Microbial Biotechnology, 00. https://doi.org/10.1111/1751-7915.14117
Escherichia coli; rod-shaped inclusion body,; inclusion body morphology; inclusion body scaffold; cell doubling; microscopy
en
Abstract:
Early-stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod-shaped protein aggregates, a potential sponge-like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod-shaped inclusion bodies, confirmed their porous morphology, the presence of multiple protein aggregates per cell and modelled inclusion body formation as function of the number of generations.
