Record link: 
http://hdl.handle.net/20.500.12708/144328
-
Title: 
Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand
en
Citation: 
Kittler, S., Ebner, J., Besleaga, M., Larsbrink, J., Darnhofer, B., Birner-Grünberger, R., Schobesberger, S., Akhgar, C. K., Schwaighofer, A., Lendl, B., & Spadiut, O. (2022). Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand. Journal of Biotechnology, 359, 108–115. https://doi.org/10.1016/j.jbiotec.2022.10.002
-
Publisher DOI: 
10.1016/j.jbiotec.2022.10.002
-
Publication Type: 
Article - Original Research Article
en
Language: 
English
-
Authors: 
Kittler, Stefan 
Ebner, Julian 
Besleaga, Mihail 
Larsbrink, Johan 
Darnhofer, Barbara 
Birner-Grünberger, Ruth 
Schobesberger, Silvia 
Akhgar, Christopher Karim 
Schwaighofer, Andreas 
Lendl, Bernhard 
Spadiut, Oliver 
-
Organisational Unit: 
E166-04 - Forschungsbereich Bioverfahrenstechnik 
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren 
E163-03-1 - Forschungsgruppe Cell Chip 
-
Journal: 
Journal of Biotechnology 
-
ISSN: 
0168-1656
-
Date (published): 
20-Nov-2022
-
Number of Pages: 
8
-
Publisher: 
ELSEVIER
-
Peer reviewed: 
Yes
-
Keywords: 
Protein L; E. coli; Recombinant production; Bioreactor; Affinity ligand
en
Abstract: 
Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.
en
Research Areas: 
Sustainable Production and Technologies: 35%
Efficient Utilisation of Material Resources: 35%
Modeling and Simulation: 30%
-
Science Branch: 
2040 - Chemische Verfahrenstechnik: 15%
1060 - Biologie: 35%
2090 - Industrielle Biotechnologie: 50%
-
Appears in Collections:
Article

Show full item record

Page view(s)

89
checked on Nov 30, 2023

Google ScholarTM

Check