Kittler, S., Ebner, J., Besleaga, M., Larsbrink, J., Darnhofer, B., Birner-Grünberger, R., Schobesberger, S., Akhgar, C. K., Schwaighofer, A., Lendl, B., & Spadiut, O. (2022). Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand. Journal of Biotechnology, 359, 108–115. https://doi.org/10.1016/j.jbiotec.2022.10.002
Protein L; E. coli; Recombinant production; Bioreactor; Affinity ligand
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Abstract:
Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.
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Research Areas:
Sustainable Production and Technologies: 35% Efficient Utilisation of Material Resources: 35% Modeling and Simulation: 30%
