|Title:||Mechanical properties of cells probed by atomic force microscopy||Language:||English||Authors:||Morozov, Dmitry||Keywords:||Atomic Force Microscopy; Force Spectroscopy; Cells; Stiffness||Advisor:||Schütz, Gerhard||Issue Date:||2020||Number of Pages:||57||Qualification level:||Diploma||Abstract:||
Atomic force microscopy (AFM) is widely used to study various biologicalobjects, among others also mammalian cells in physiologically adequate conditions. Force-distance curves provide information about mechanical characteristics of the cell, such as contact stiffness, deformation, Young's modulus etc. Such properties have been linked to various states of disease. AFM studies of the morphology and mechanical properties of native cells can be used in biomedical applications, such as determining the physiological and pathological states of cells. In this work, AFM was applied to examine the mechanical properties of the human umbilical vein endothelial cells (HUVEC) and their dependence on different measurement settings. The influence of the loading rate, maximum load and indenter geometry on the measurement of mechanical properties of such cells has been studied. By applying a linear fitting to a Force-Distance-Curves obtained during nanoindentation experiments the stiffness and the Young’s modulus were calculated. Relation of the mechanical properties of the cells and the experiment condition was demonstrated. Obtained results can be used in a further studies to compare viscoelastic properties of the approach and the retracting curve.
|DOI:||10.34726/hss.2020.85180||Library ID:||AC16072849||Organisation:||E134 - Institut für Angewandte Physik||Publication Type:||Thesis
|Appears in Collections:||Thesis|
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