Title: Enhancing cell packing in buckyballs by acoustofluidic activation
Authors: Ren, Tanchen 
Steiger, Wolfgang 
Chen, Pu 
Ovsianikov, Aleksandr  
Demirci, Utkan  
Category: Original Research Article
Keywords: networks of neurons
Issue Date: 31-Mar-2020
Journal: Biofabrication 
Abstract: 
How to pack materials into well-defined volumes efficiently has been a longstanding question of interest to physicists, material scientists, and mathematicians as these materials have broad applications ranging from shipping goods in commerce to seeds in agriculture and to spheroids in tissue engineering. How many marbles or gumball candies can you pack into a jar? Although these seem to be idle questions they have been studied for centuries and have recently become of greater interest with their broadening applications in science and medicine. Here, we study a similar problem where we try to pack cells into a spherical porous buckyball structure. The experimental limitations are short of the theoretical maximum packing density due to the microscale of the structures that the cells are being packed into. We show that we can pack more cells into a confined micro-structure (buckyball cage) by employing acoustofluidic activation and their hydrodynamic effect at the bottom of a liquid-carrier chamber compared to randomly dropping cells onto these buckyballs by gravity. Although, in essence, cells would be expected to achieve a higher maximum volume fraction than marbles in a jar, given that they can squeeze and reshape and reorient their structure, the packing density of cells into the spherical buckyball cages are far from this theoretical limit. This is mainly dictated by the experimental limitations of cells washing away as well as being loaded into the chamber.
DOI: 10.1088/1758-5090/ab76d9
ISSN: 1758-5082
Organisation: E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication 
License: CC BY 4.0 CC BY 4.0
Publication Type: Article
Artikel
Appears in Collections:Article

Files in this item:

File Description SizeFormat
Ren_2020_Biofabrication_12_025033.pdfPublished version1.11 MBAdobe PDFThumbnail
 View/Open
BF025033_suppdata.pdfSupplementary data600.61 kBAdobe PDFThumbnail
 View/Open
Show full item record

Page view(s)

20
checked on Feb 26, 2021

Download(s)

16
checked on Feb 26, 2021

Google ScholarTM

Check


This item is licensed under a Creative Commons License Creative Commons