Evaluating a robust and easy-to-use biological-activity-based method to assess the presence of potentially adverse bacteria at two riverbank filtration sites along the Danube river: A case study

Abstract

The Laboratory Biological Activity Reaction Test (LAB-BART) is an easy-to-use assay that utilizes metabolic capabilities to process an array of substrates to semi-quantitatively assess the presence of potentially adverse bacteria in a groundwater sample. Here, we evaluated LAB-BART for the assessment of groundwater samples obtained under real-life conditions from two riverbank filtration (RBF) sites in Austria. Samples were taken monthly for an overall experimental period of six months and analyzed following the manufacturer’s recommendations for measuring iron-related, sulfate-reducing, slime-forming and denitrifying bacteria. Additional measurements were done for analyzing chemical water composition, as well as bacterial community structure to evaluate the suitability of LAB-BART by identifying relevant bacteria. Results imply that while LAB-BART could not give detailed information on bacterial concentrations, it might be able to indicate hydrologically induced changes in biogeochemical processes in a subsurface system, thus allowing operators to determine an adequate response to a potential influx of undesired bacteria. Despite its limitations, LAB-BART might therefore be a valuable tool for monitoring purposes due to its ease of use, but more research is necessary to determine its accuracy in measuring bacterial activity.