Szabadi, A., Klausser, R., Spadiut, O., & Schröder, C. (2023). Inclusion Bodies in Ionic Liquids. Liquids, 4(1), 1–31. https://doi.org/10.3390/liquids4010001
inclusion bodies; ionic liquids; mild solubilization; protein folding
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Abstract:
The pivotal role of proteins in pharmaceuticals is challenged by stability issues, making the study of inclusion bodies—a source of insoluble protein aggregates—increasingly relevant. This review outlines the critical procedures in inclusion body processing, focusing on ’mild solubilization concepts’ and refolding methodologies. Attention is afforded to the emerging role of ionic liquids with unique and tunable physicochemical properties in optimizing protein unfolding and refolding processes. The review critically assesses the existing literature at the intersection of inclusion bodies and ionic liquids, identifying recent advancements, potential applications, and avenues for future research. This comprehensive analysis aims to elucidate the complexities in efficient protein processing from inclusion bodies.
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Project title:
CD Labor für Inclusion Body Prozessierung 4.0: CDL IB4.0 (Christian Doppler Forschungsgesells)
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Research Areas:
Biological and Bioactive Materials: 50% Sustainable Production and Technologies: 50%
