Klausser, R. (2023, November 14). Analysis of cell lysis efficiency and inclusion body size distribution via centrifugal sedimentation [Poster Presentation]. PEGS Europe: Protein and Antibody Engineering Summit 2023, Lisbon, Portugal.
PEGS Europe: Protein and Antibody Engineering Summit 2023
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Event date:
14-Nov-2023 - 16-Nov-2023
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Event place:
Lisbon, Portugal
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Keywords:
Inclusion Body; centrifugation; cell lysis
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Abstract:
Differential centrifugal sedimentation is investigated as a method of measuring inclusion body size distribution and estimating the efficiency of cell lysis via high pressure homogenization. Comparing the results obtained via sedimentation with microscopic measurements suggests an influence of the lysis conditions on the inclusion body density. By comparing the normalized relative weights of inclusion bodies and remaining cells, the degree of cell lysis could be estimated via sedimentation
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Project title:
CD Labor für Inclusion Body Prozessierung 4.0: CDL IB4.0 (Christian Doppler Forschungsgesells)
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Research Areas:
Biological and Bioactive Materials: 50% Efficient Utilisation of Material Resources: 50%
