Tendinopathies are becoming more prevalent, yet their exact cause is not always clear. It is hypothesized that inflammation plays a crucial role in extracellular matrix remodeling and therefore, compromises the mechanical and biological characteristics of tendons. The lack of a tendon tissue model makes it difficult to verify this hypothesis. A universal and approved in vitro tendon model that closely resembles the physiological characteristics of tendons currently is needed. Tendon models usually rely on mechanical stimulation, which requires the determination of stimulation parameters alongside scaffold selection, characterization, and optimization, to induce cellular alignment and production of proper ECM. Here, we employed a tension-based MagneTissue bioreactor to establish a tendon-like tissue model by controlled mechanical stimulation of tendon-derived progenitor cells (TDPCs) embedded in fibrin constructs. Briefly, 3D fibrin rings were seeded with TDPCs. Cells were cultivated in a growth medium and mechanical strain in two different ways (static load and cyclic load) was subsequently applied. Cell viability, and alignment as well as their localization and density within the construct were analyzed 7 days after stimulation using immunofluorescent staining. Cells in both cell lines were viable and aligned towards the axis of strain. However, the distribution of cells throughout the scaffold varied. In both groups, cells were predominantly located on the surface and there was no difference in cell number. Although, there was a tendency to have more cells inside in the group of cyclic load compared to both the static load and non-loaded groups. Additionally, cyclic-loaded samples showed fewer fragmented nuclei inside the construct, which might indicate a lower level of apoptosis. We can assume that cyclic loading appears to improve media penetration and cell survival and it is a crucial stimulation parameter to generate tendon-like constructs.