Three-dimensional (3D) in-vitro cell culture is an appropriate tool that closely mimics the behavior of in-vivo tissues. It can replicate the cell's growth rate, metabolic activity, cell-cell interaction and cell-substrate interaction more accurately than the standard two-dimensional cell culture. In this project, we combined the benefits of Electrical Impedance Spectroscopy (EIS) as a noninvasive and label-free monitoring technique, with 3D Tumor Spheroid (3D-TS) on a Microelectrode Array (MEA) to develop a bioelectronic platform for preclinical drug development. 3D-TS of roughly 300 ìm size made of a human colon cancer cell line were cultured on planar MEA comprising 60 electrodes. Control and drug treatment experiments were conducted using EIS in the frequency range of 100 Hz-1MHz to observe the 3d-TS response over time. After interfacing the 3D-TS with the MEA, we obtained significantly higher impedance amplitudes for electrodes underneath the 3D-TS than for bare electrodes. During the drug treatment, EIS revealed a time-dependent reduction in the impedance relative to the control groups. In conclusion, our platform allows for reliable, noninvasive recording from inside of 3D-TS without disaggregating the cells. This achievement allows simple preclinical drug screening applications.