Biomass gasification using dual fluidized bed (DFB) steam gasification allows the production of a high-quality product gas that can be used for a variety of synthesis processes. During the last years extensive research has been conducted on the 100 kW pilot plant of TU Wien and the main application areas of methanation, Fischer-Tropsch-processes and hydrogen production. Since the production of bio-based chemicals is gaining more and more importance new application fields are researched. The concept of this paper consists of combining DFB steam gasification, gas cleaning and syngas fermentation in order to directly produce acetate from softwood pellets. For syngas fermentation the strictly anaerobic acetogenic bacteria T. kivui were used. T. kivui is able grow on three of the four main gas components of the DFB product gas, namely on H2, CO2 and CO. Only CH4 acts as an inert gas in the syngas fermentation reactor. Various pre-tests had to be carried out before finally the three process steps could be connected. Pre-tests were conducted with bottled syngas collected after the final cleaning step. After successful adaptation of T. kivui to the syngas small scale experiments were carried out to gain knowledge about operating parameters, yields and potential problems of the process. For a total of 25 hours purified DFB syngas was fed to the fermentation reactor. The DFB product gas was cleaned in a hot gas filter, a RME scrubber, two activated carbon beds and a ZnO-bed. Measurements at the beginning and the end of the experiment confirm that contaminations from DFB steam gasification remained constant over time. Since syngas was produced directly the bacteria had to the deal with fluctuations in the gas composition and short periods with lack of gas supply. Neither of them affected the growth of the bacteria negatively which makes the combination of the three process steps a very robust overall concept. Additionally, the impact of impurities originating from the real DFB steam gasification process proved not be harmful to T. kivui. This was demonstrated by contaminating the environment of T. kivui with water condensed in the RME scrubber. Since this water contains ammonia, H2S, water-soluble organic compounds and particles certain gas cleaning steps may be superfluous for syngas fermentation making the overall concept simpler and more economic.