Saghafi, S., Foroughipour, S. M., Foroughipour, M., Prado-López, S., Oakes, J., & Becker, K. (2025). Beam width tunability using refractive optics in static light sheet microscopy. In K. Fliegel & V. Prajzler (Eds.), Photonics, Devices, and Systems IX. SPIE. https://doi.org/10.1117/12.3072732
This study presents a tunable light sheet microscope for high-resolution three-dimensional biological imaging. The core innovation integrates aspheric lenses with a single Powell lens in the optical pathway. The Powell lens introduces a phase shift that creates a bone-shaped beam profile, reducing the central intensity hot spot and achieving more uniform intensity distribution. Simulations and experimental results confirm this optical configuration generates thin, high-quality light sheets. Optimal performance occurs with Powell lenses having fan angles of 5°-10° and tip radii of 0.1-0.4 mm. The system demonstrates advanced imaging capabilities through visualization of various biological specimens. This establishes the microscope as a powerful tool for high-resolution studies of tissues, neural networks, and vascular systems in fundamental research and medical applications.
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Project title:
Gewebereinigung und Beschriftung mit Megasound: NXT22-001 (WWTF Wiener Wissenschafts-, Forschu und Technologiefonds) Bildgebung von Krebs mit zellulärer Auflösung durch eine Einheit für gerichtetes Licht ausgewählter Wellenlängen - DLSSWU: P2403385-W3G01 (Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftu)
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Research Areas:
Biological and Bioactive Materials: 20% Modeling and Simulation: 30% Computational System Design: 50%
