Title: Fast quantification of bovine milk proteins employing external cavity-quantum cascade laser spectroscopy
Language: English
Authors: Schwaighofer, Andreas 
Kuligowski, Julia
Quintas, Guillermo 
Mayer, Helmut K. 
Lendl, Bernhard  
Keywords: External cavity-quantum cascade laser (ECQCL); Total protein; Casein; β-lactoglobulin; Bovine milk analysis; Chemometrics
Issue Date: 2018
Journal: Food Chemistry
Analysis of proteins in bovine milk is usually tackled by time-consuming analytical approaches involving wet-chemical, multi-step sample clean-up procedures. The use of external cavity-quantum cascade laser (EC-QCL) based IR spectroscopy was evaluated as an alternative screening tool for direct and simultaneous quantification of individual proteins (i.e. casein and β-lactoglobulin) and total protein content in commercial bovine milk samples. Mid-IR spectra of protein standard mixtures were used for building partial least squares (PLS) regression models. A sample set comprising different milk types (pasteurized; differently processed extended shelf life, ESL; ultra-high temperature, UHT) was analysed and results were compared to reference methods. Concentration values of the QCL-IR spectroscopy approach obtained within several minutes are in good agreement with reference methods involving multiple sample preparation steps. The potential application as a fast screening method for estimating the heat load applied to liquid milk is demonstrated.
DOI: 10.1016/j.foodchem.2018.01.082
Library ID: AC15518674
URN: urn:nbn:at:at-ubtuw:3-7520
ISSN: 0308-8146
Organisation: E164 - Institut für Chemische Technologien und Analytik 
License URI: https://creativecommons.org/licenses/by-nc-nd/4.0/deed.de
Publication Type: Article
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