Title: | Fast quantification of bovine milk proteins employing external cavity-quantum cascade laser spectroscopy | Language: | English | Authors: | Schwaighofer, Andreas Kuligowski, Julia Quintas, Guillermo Mayer, Helmut K. Lendl, Bernhard ![]() |
Keywords: | External cavity-quantum cascade laser (ECQCL); Total protein; Casein; β-lactoglobulin; Bovine milk analysis; Chemometrics | Issue Date: | 2018 | Journal: | Food Chemistry | Abstract: | Analysis of proteins in bovine milk is usually tackled by time-consuming analytical approaches involving wet-chemical, multi-step sample clean-up procedures. The use of external cavity-quantum cascade laser (EC-QCL) based IR spectroscopy was evaluated as an alternative screening tool for direct and simultaneous quantification of individual proteins (i.e. casein and β-lactoglobulin) and total protein content in commercial bovine milk samples. Mid-IR spectra of protein standard mixtures were used for building partial least squares (PLS) regression models. A sample set comprising different milk types (pasteurized; differently processed extended shelf life, ESL; ultra-high temperature, UHT) was analysed and results were compared to reference methods. Concentration values of the QCL-IR spectroscopy approach obtained within several minutes are in good agreement with reference methods involving multiple sample preparation steps. The potential application as a fast screening method for estimating the heat load applied to liquid milk is demonstrated. |
DOI: | 10.1016/j.foodchem.2018.01.082 | Library ID: | AC15518674 | URN: | urn:nbn:at:at-ubtuw:3-7520 | ISSN: | 0308-8146 | Organisation: | E164 - Institut für Chemische Technologien und Analytik | Publication Type: | Article Artikel |
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