Alcaráz, M. R., Schwaighofer, A., Goicoechea, H., & Lendl, B. (2017). Application of MCR-ALS to reveal intermediate conformations in the thermally induced α-β transition of poly-l-lysine monitored by FT-IR spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 185, 304–309. https://doi.org/10.1016/j.saa.2017.05.005
Temperature-induced conformational transitions of poly-L-lysine were monitored with Fourier-transform infrared (FT-IR) spectroscopy between 10 °C and 70 °C. Chemometric analysis ofdynamic IR spectra was performed by multivariate curve analysis–alternating least squares (MCR-ALS) ofthe amide I′ and amide II′ spectral region. With this approach, the pure spectral and concentration profiles ofthe conformational transitionwere obtained. Beside the initial α-helical, the intermediate random coil/extended helices and the final β-sheet structure, an additional intermediate PLL conformation was identified and attributed to a transient β-sheet structure.
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The final publication is available via <a href="https://doi.org/10.1016/j.saa.2017.05.005" target="_blank">https://doi.org/10.1016/j.saa.2017.05.005</a>.
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