FTIR spectroscopy as a novel analytical approach for investigation of glucose transport and glucose transport inhibition studies in transwell in vitro barrier models

Abstract

Glucose transport is key for cellular metabolism as well as physiological function and is maintained via passive facilitated and active sodium-glucose linked transport routes. Here, we present for the first time Fouriertransform infrared spectroscopy as a novel approach for quantification ofapical-to-basolateral glucose transport ofin vitro cell barriermodels using liver, lung, intestinal and placental cancer cell lines. Results ofour comparative study revealed that distinct differences could be observed upon subjection to transport inhibitors.