Dimitrijevic, N. (2023). Evaluation and testing of a self-filling particle trapping microfluidic chip [Diploma Thesis, Technische Universität Wien]. reposiTUm. https://doi.org/10.34726/hss.2023.97521
The ability to trap single cells inside a microfluidic chip and subsequent imaging is normally paired with the use of a precise and expensive external pressure source to control the liquid flow. Here we present a self-filling particle trapping chip, which can be used without additional equipment. Different designs of hydrodynamic cell traps were evaluated using cell sized beads and Jurkat T cells. Also, how different plasma treatment conditions of the PDMS surface combined with accelerated aging influence the trapping ability of the chips was investigated. The cell-trapping and self-filling function of the chip could be validated but optimisations of the design are recommended to increase the efficiency.
