Record link: 
http://hdl.handle.net/20.500.12708/223334
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Title: 
Adapting Komagataella phaffii Cultivation Media to REACH Regulations
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Citation: 
Kopp, J., Pekarsky, A., & Spadiut, O. (2025). Adapting Komagataella phaffii Cultivation Media to REACH Regulations. In X. Garcia-Ortega, A. Glieder, K. Kovar, & L. Rieder (Eds.), Pichia pastoris : Methods and Protocols (Vol. 2697, pp. 445–455). Humana. https://doi.org/10.1007/978-1-0716-4779-0_30
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Publisher DOI: 
10.1007/978-1-0716-4779-0_30
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Publication Type: 
Book Contribution - Edited Volume Contribution
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Language: 
English
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Authors: 
Kopp, Julian 
Pekarsky, Alexander 
Spadiut, Oliver 
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Organisational Unit: 
E166-04-2 - Forschungsgruppe Integrierte Bioprozessentwicklung 
E166-04-1 - Forschungsgruppe Bioprozess-Technologie 
E056-12 - Fachbereich ENROL DP 
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Published in: 
Pichia pastoris : Methods and Protocols 
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ISBN: 
978-1-0716-4779-0
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Date (published): 
1-Oct-2025
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Number of Pages: 
11
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Publisher: 
Humana, New York
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Peer reviewed: 
Yes
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Keywords: 
Bioreactors; Cobalt; Boric Acids; Recombinant Proteins; Boron; Boron leakage; Cobalt; Fermentation; Glass vessel; Komagataella phaffii; Pichia pastoris; REACH; Stainless steel; boron; Culture Media; Saccharomycetales
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Abstract: 
REACH stands for "Registration, Evaluation, Authorization, and Restriction of Chemicals" and classifies certain substances as harmful to human health and the environment. Boric acid and cobalt dichloride, commonly used as boron (B) and cobalt (Co) sources in cultivation media for Komagataella phaffii, represent such harmful substances. However, B and Co support cellular growth, and yeast harbors a large number of co-containing enzymes. Even though cell physiology (up to 90 g/L) was not affected by the removal of boric acid and cobalt dichloride from the media, recombinant protein production varied when excluding these components from the cultivation media to comply with REACH regulations. Here, we describe how to analyze and potentially adapt a defined cultivation medium for K. phaffii bioreactor cultivations concerning current REACH regulations.
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Research Areas: 
Metallic Materials: 20%
Biological and Bioactive Materials: 20%
Sustainable Production and Technologies: 60%
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Science Branch: 
2040 - Chemische Verfahrenstechnik: 50%
1060 - Biologie: 35%
2090 - Industrielle Biotechnologie: 15%
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Appears in Collections:
Book Contribution

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