Biocompatible a-SiC:H-Based Bistable MEMS Membranes With Piezoelectric Switching Capability in Fluids

Abstract

In this paper, we demonstrate biocompatible micromachined buckled membranes for the operation in liquids. The membranes feature diameters between 600 and 800 μm as well as integrated piezoelectric thin film actuators, thus enabling switching between the bistable states. The membrane material is known to be not biocompatible, hence a hydrogenated amorphous silicon carbide (a-SiC:H) layer is deposited on the surface. For demonstration purposes, a 70 nm ±3 nm thin a-SiC:H coating with a specific silicon to carbon ratio was chosen, with a negligible impact on the overall switching performance of the bistable membranes. Furthermore, a relation between the membrane center velocity at the first characteristic resonance frequency and the switching ability of a membrane in different viscous fluids is shown. Based on a small signal analysis the switching behavior can be predicted. The membranes were successfully switched in liquids with a dynamic viscosity up to 286 mPa s. The biocompatibility of the membranes was examined by growing Caco-2 cells, a human carcinoma cell line, on a-SiC:H thin films, featuring different carbon contents and organic surface treatments. The proliferation and adhesion of the cells on the substrates are examined in an empirical cell growth and removal study. Only a-SiC:H surfaces pre-treated with an O2-plasma and coated with Collagen Type I indicated to provide an environment of improved cell adhesiveness compared to other surface treatments. The biological investigations resulted in good cell proliferation, that also depends on the altered hydrophilicity of the surface, as well as on the carbon content of the a-SiC:H thin films. This study reveals that a broad range of biocompatible a-SiC:H surfaces can be prepared, whereby the cell growth can be tailored in terms of proliferation and adhesion for different biomedical application scenarios. Finally, this paper reports on the mechanical features of bistable, buckled membranes and their suitability as a growth substrate for human cell cultures, due to the good biocompatibility of a-SiC:H thin films. We therefore suggest that it will be feasible to grow cells on bistable MEMS membranes, enabling cell experiments in liquid medical environments, with both mechanically excitable and biocompatible surfaces. [2022-0006].