Record link: 
http://hdl.handle.net/20.500.12708/144517
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Title: 
Monomeric TCRs Drive T Cell Antigen Recognition
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Citation: 
Brameshuber, M., Kellner, F., Rossboth, B., Ta, H., Alge, K., Sevcsik, E., Göhring, J., Axmann, M., Baumgart, F., Gascoigne, N. R. J., Darvis, S. J., Stockinger, H., Schütz, G., & Huppa, J. B. (2018). Monomeric TCRs Drive T Cell Antigen Recognition. Nature Immunology, 19(5), 487–496. https://doi.org/10.1038/s41590-018-0092-4
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Publisher DOI: 
10.1038/s41590-018-0092-4
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Publication Type: 
Article - Original Research Article
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Language: 
English
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Authors: 
Brameshuber, Mario 
Kellner, Florian 
Rossboth, Benedikt 
Ta, Haisen 
Alge, Kevin 
Sevcsik, Eva 
Göhring, Janett 
Axmann, Markus 
Baumgart, Florian 
Gascoigne, Nicholas R.J. 
Darvis, Simon J. 
Stockinger, Hannes 
Schütz, Gerhard 
Huppa, Johannes B. 
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Organisational Unit: 
E134-04 - Forschungsbereich Biophysics 
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Journal: 
Nature Immunology 
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ISSN: 
1529-2908
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Date (published): 
May-2018
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Number of Pages: 
10
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Publisher: 
NATURE PORTFOLIO
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Peer reviewed: 
Yes
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Keywords: 
Immunology; Immunology and Allergy
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Abstract: 
T cell antigen recognition requires T cell antigen receptors (TCRs) engaging MHC-embedded antigenic peptides (pMHCs) within the contact region of a T cell with its conjugated antigen-presenting cell. Despite micromolar TCR:pMHC affinities, T cells respond to even a single antigenic pMHC, and higher-order TCRs have been postulated to maintain high antigen sensitivity and trigger signaling. We interrogated the stoichiometry of TCRs and their associated CD3 subunits on the surface of living T cells through single-molecule brightness and single-molecule coincidence analysis, photon-antibunching-based fluorescence correlation spectroscopy and Förster resonance energy transfer measurements. We found exclusively monomeric TCR- CD3 complexes driving the recognition of antigenic pMHCs, which underscores the exceptional capacity of single TCR-CD3 complexes to elicit robust intracellular signaling.
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Research Areas: 
Materials Characterization: 50%
Biological and Bioactive Materials: 50%
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Science Branch: 
Physik, Astronomie
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